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When combined with the widely used next-generation RNA sequencing methods, such research methods should enable an increased understanding of the possible pathways underlying depression (205)

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One intriguing aspect of this balance is the relationship between glutathione, a tripeptide composed of glutamine, cysteine, and glycine, and uric acid, a compound produced during the breakdown of purines

Quantitative real-time PCR (qRT-PCR) and Western blot assay Total RNA was extracted using Trizol reagent (Invitrogen) according to the manufacturers instructions protocol

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( a ) Schematic of Rosa26 targeting and the Hmgcs2 iOE construct