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In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging

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Convert vial amount to micrograms (if needed): mg 1,000 = mcg Concentration: total amount (mcg) total liquid (mL) = mcg/mL Draw volume: target amount (mcg) concentration (mcg/mL) = mL U100 units: mL 100 = syringe units Worked example (math only) If a vial is labelled 50 mg (50,000 mcg) and you add 10 mL, the concentration is 5,000 mcg/mL

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