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2.13 ACE and ACE2 activities To assess angiotensin-converting enzyme (ACE) and ACE2 activity measurement, kidney protein extracts were obtained from kidney cortex homogenized with lysis buffer (50 mM HEPES pH7.4, 150 mM NaCl, 0.5% Triton-X-100, 0.025 mM ZnCl 2 , 0.1 mM Pefabloc SC Plus (11873601001, Roche) and a 1:7 dilution of EDTA-free protease inhibitor cocktail (11836170001, Roche))

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(H) Immunoblot blot analysis of -smooth muscle actin (SMA), phosphorylated Yes-associated protein (pYAP, Ser127), total YAP, and transcriptional coactivator with PDZ-binding motif (TAZ) proteins

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Current treatments include injectable GLP-1 agonists like semaglutide

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Direct answer: If tirzepatide burps keep happening, track the symptoms and review them with a qualified clinician

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