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Hippocampal neurons were then cultured a further 3-7 days, at which time they were either transfected or treated with various pharmacological reagents as described in (Wayman et al., 2008)
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GraphPad Prism version 8.0 (GraphPad Software, Inc., San Diego, California, USA) was used to create the figures
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The positioning of your upper back and neck can also affect the functioning of your shoulder so they will also observe this