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U2OS cells were cultured in McCoys 5A with 10% FCS (Gibco, Invitrogen) and 1% glutamax, 0.5 g/ml hydrocortisone

Charged NAD+ molecules are then driven by the electric field, penetrating the skin barrier directly and entering the subcutaneous tissues for efficient absorption

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However, subsequent research revealed that HGF and its receptor c-Met are widely expressed in the brain, particularly in the hippocampus, cortex, and cerebellum

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