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Positive deletion- and reconstituted- strains were screened by Southern analysis (Figure S1) and DIG-hybridisation probes were generated using primers ogliT-5 and ogliT-4

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Rapid mitochondrial purification for metabolite profiling Mitochondria were purified from HEK-293T, HeLa, or K562 cells expressing 3xHA-OMP25-mCherry (mitochondrial isolation) or 3xMyc-OMP25-mCherry (background control) according to a previously described protocol 40

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