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The PCR program was set as: 98 C for 3 min, 98 C for 10 s, 5560 C (temperature and time were set according to primers), 72 C for 30 s, and a total of 35 cycles

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A The difference in symptom severity may be due to genetic and biological differences, such as the structure of red blood cells, metabolism, biochemistry, and testosterone levels

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