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After cell dispensing, plates were centrifuged at 500 g for 2 min and incubated at 37 C and 5% CO 2 for 4 h

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In addition, mtROS can also directly act on some proteins on the mitochondrial matrix or mitochondrial membrane, and regulate the activity of mitochondrial complex I, complex III, and complex IV through Toll-like receptors, retinoic acid-inducible gene I-like receptors and other signaling pathways, thereby affecting OXPHOS