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Two complementary libraries were synthesized with either fixed positions p7 (A) or p8 (Y) and every other amino acid (excluding Cys, Asp and Glu) were tested at p8 or p7, respectively

FIGURE 2 FIGURE 3 FIGURE 4 Validation of selected metabolite biomarkers through selected reaction monitoring Figure 5 depicts the quantification results of acetylcarnitine selected as the final biomarker candidate in the validation set