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At each time point (five minutes intervals), photoluminescence spectra was recorded using a FluoroMax 4 (Horiba, Jobin Yvon GmbH, Unterhaching, Germany) spectrophotometer working with a continuous 450 W xenon lamp at ex / em of: 365/605, 552/636 and 495/519 nm for PLGA encapsulating DDA-GSH-AuNCs, Nile red or fluorescein, respectively