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In vitro ubiquitylation assays All ubiquitylation assays were performed at room temperature in the final reaction buffer containing 25 mM HEPES pH 7.5, 150 mM NaCl, 0.25 mM DTT, 5 mM ATP and 10 mM MgCl 2

The freeze-thaw cycle can potentially damage the lyophilized cake structure, making reconstitution slightly more difficult without affecting actual peptide integrity

The peptides you're dissolving into it are not

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Development of poly (2-oxazoline) and poly (2-oxazine) based hydrogels for additive

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