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Hansen et al [17] used Hanks Balanced Salt Solution (HBSS) containing 5% FCS and 2 mM EDTA for both, while Cervantes-Barragan et al [15] replaced the HBSS with PBS and Luckashenak et al [16] used PBS for suspending the cells and HBSS for diluting the OptiPrep (both containing 0.2% BSA and 2 mM EDTA

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CTGF enhances migration and MMP-13 up-regulation via v3 integrin, FAK, ERK, and NF-B-dependent pathway in human chondrosarcoma cells

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With respect to the requirement for Mg 2+ in ATP functions, essentially all of the ATP in the cell has Mg 2+ bound to the phosphates

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