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4- Always keep the water level touching the bottom of the grid

When symptoms strike, many people wonder if antibiotics are the only way to get relief

HepG2 cells was cultured in Dulbeccos modified Eagles medium (DMEM, Gibco) supplemented with 10% (v/v) fetal bovine serum (Hyclone) and antibiotics (100 U/mL penicillin and 100 U/mL streptomycin) (Hyclone) at 37 C in a humidified atmosphere containing 5% CO2

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Future studies aiming to include broader patient demographics and additional biological markers could further refine these therapeutic approaches