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The in vitro experiments were performed using TM3 mouse Leydig cells that were cultured in DMEM/F-12 medium supplemented with 5% horse serum and 2.5% fetal bovine serum, and maintained at 37C under a 5% CO2 atmosphere

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The BMEC monolayers were mounted in a side-by-side diffusion apparatus and the transendothelial flux of DSIP analysed by HPLC with UV detection at 280 nm