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After digestion of pUC57 vectors with EcoRI and AgeI restriction enzymes, corresponding DNA fragments were ligated into the pLVX-TetOne-Puro vector (Addgene, Plasmid #124797)

10.1016/j.coemr.2021.100285 154

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GHK-Cu drives collagen synthesis signaling, but that signaling requires adequate amino acid substrate to produce actual collagen

This is believed to result from the formation of a reactive metabolite, N -acetylhydrazine, which is produced by acetylation followed by oxidative metabolism

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