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Cryosections staining and microscopy Hematoxylin and eosin (H&E) and immunofluorescence of tissues were performed on snap-frozen tissue samples in O.C.T

The Nissen procedure involves a complete wrap, while the Toupet technique uses a partial, 270-degree wrap to offload the esophagus

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TCs exposure leads to apoptosis both and in several organs such as lymphoid organs, hematopoietic tissues, liver, intestinal crypts, bone marrow and thymus

Acknowledgments This work was supported by the Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine

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