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This experiment was also performed to confirm if the high concentration of the reduction reagent can affect the separation and change the shape of the N-Hcy-Lys signal

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These results in the conversion of NO to various reactive nitrogen species which include nitrosonium cation (NO + ), nitroxyl anion (NO ) and peroxynitrite (ONOO ) (Table 2)

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First, I want to note that a sufficient supply of protein and, above all, essential amino acids , which the body cannot produce itself, is critical for good health