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We observed upregulation of the stemness markers CD133 and CD44 in the CRC DTP cells ( Figure 2E )

Furthermore, we confirm that the initial glutathionylation of the glutaredoxin moiety and not the glutathione transfer to the roGFP2 moiety is rate-limiting for the oxidation of fusion constructs at physiological or low micromolar GSSG concentrations, thus allowing glutaredoxin structure-function analysis in vivo

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