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Alternatively, GSH can be measured in samples not pre-treated with NEM as total GSH (tGSH, i.e., GSH+ GSSG) by the GSH-recycling method [35]

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There may be some merit in the use of this solution as one of its components, 5.7% (w/v) polysucrose, may cause the residual erythrocytes to aggregate further and thus assist their sedimentation into a pellet