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Step 4: Insert the needle at a 45 to 90 degree angle, depending on the thickness of the skin fold

Journal of pharmaceutical and biomedical analysis, 2008, 47(3): 596- 602

Wound healing

[2] First identified in the 1970s, it has attracted scientific interest for its apparent roles in tissue repair, wound healing, and cellular regeneration

Wakabayashi T, Yamaguchi K, Matsui K, Sano T, Kubota T, Hashimoto T, Mano A, Yamada K, Matsuo Y, Kubota N, Kadowaki T, Iwatsubo T (2019) Differential effects of diet- and genetically-induced brain insulin resistance on amyloid pathology in a mouse model of Alzheimers disease

Consistency is key