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Luminescence was recorded for 45 min at 1 min intervals using a Fluoroskan Ascent FL (Thermo Scientific, Rockford, IL, USA) fluorimeter (excitation: 538 nm, emission: 604 nm), and the slopes were used for calculations
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Disc electrophoresis-II
Available from: Vitamin B12 Factsheet for Professionals, National Institutes of Health, Vitamin B12 - Uses, Side Effects, and More, WebMD, Okawa M, Mishima K, Nanami T, et al
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