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Adjust based on response, not impatience

HUBERMAN: Before we begin, I'd like to emphasize that this podcast is separate from my teaching and research roles at Stanford

Prince provides guidance on maintaining the benefits and suggests an appropriate maintenance schedule based on individual needs

Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations

The Immediate Shift: Week 1 The first week is often deceptive

PubMed, Zhang, Yu, et al