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Withaar C, Meems LMG, Markousis-Mavrogenis G, Boogerd CJ, Sillje HHW, Schouten EM, et al

It is involved in nearly 60 enzyme systems in the body, necessary for normal growth, red blood cell synthesis, and vitamin B12 absorption

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Fornaro M et al

Materials and methods Cell lines and reagents The MCF-7 human breast cancer cell line was obtained from Dr Dean Edwards (University of Texas, San Antonio, TX, USA) and was maintained in phenol red RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/mL penicillin, 100 g/mL streptomycin, 1 non-essential amino acids and bovine insulin at 6 ng/mL