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For immunoblotting, membranes were blocked in 5% skim milk powder and 1% BSA in PBST for 1 h and incubated in anti-5-hydroxymethylcytosine antibody

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Similarly, the criterion for assessing nutritional essentiality of amino acids for humans was a short-term (8-day) nitrogen balance in healthy young adults (Rose 1957)

Using the articularis genu to test peri-articular muscle health during knee osteoarthritis

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