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Refinement was performed using Phenix 54 and Refmac5 55,56 , coupled with rounds of manual model building, real-space refinement and structure validation performed using COOT 57

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Total body water was determined with bioelectrical impedance spectroscopy (TBF-410GS, Tanita Inc., Arlington Heights, IL, USA) using a low energy, high frequency current (500 micro amps at a frequency of 50 kHz)