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For example, cancer cells frequently experience glutamine famine in the body as a result of their rapid glutamine consumption

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Then the gel incubated in a mixture containing 50 mM TrisHCL (pH 7.5), 0.55 mg/ml NBT, 0.5 m nicotinamide adenine dinucleotide phosphate (NADP), 2 mM MnCl 2 , 18 mM malate and 0.1 mg/ml phenazine methosulfate (PMS) for 2 h

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