After blocking of endogenous peroxidase, sections were incubated in immunoblocking buffer (5% normal donkey serum and 0.2% Triton-X in PBS) for 1 h, followed by overnight incubation in primary antibody rabbit anti-cFos (1:1000, Cell Signaling, Danvers, USA)
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Casein makes up about 40% of mature milk protein and is suspended in micelles for improved digestion (increased surface for enzymatic digestion.) Whey is a fast digesting milk protein that makes up about 60% of mature milk protein
HCG is administered via a small subcutaneous injection (just beneath the skin), typically using a very fine, short needle similar to an insulin syringe
Complexation Following synthesis, the peptide is complexed with copper(II) ions under controlled conditions to form GHK-Cu
