Briefly, to separate epidermis from dermis, whole skin from mice at postnatal day (P)0-P3 were incubated in a solution of 5 mg/ml Dispase II (#D4693, Sigma-Aldrich, USA) diluted in RPMI medium (Gibco, USA) supplemented with 10% fetal calf serum (FCS, #S0615, Sigma-Aldrich, USA), penicillin (100 U/ml), streptomycin (100 g/ml), 100 nM sodium pyruvate, and 10 mM non-essential amino acids (all Gibco, USA) at 4 C overnight
our priorities were to not pack too many activities where we would become exhausted & overwhelmed, but to chill & just enjoy ourselves in hindsight, i think our itinerary was close to perfect bcos we ended up a BEST LOW COMMITMENT FREELANCE JOBS
The easily accessible dermis is silently deconstructed by UV rays leading to solar elastosis
Anawalt, A
Drger O, Gottschalk J, Wischmeyer E, et al
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