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A dominant-negative Gs (DNGs) was generated 3 by introducing eight mutations: S54N, G226A, E268A, N271K, K274D, R280K, T284D, and I285T

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This FDA-approved medication works on both GLP-1 and GIP pathways

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In addition, a non-competitive ECD-binding antibody, Fab fragment (Fab7F38) 17 , was added along with the TMD-binding negative allosteric modulator (NAM) PF-06372222 (originally designed for GCGR) 18 for co-crystallization and successful determination of the structure to 3.2 resolution (Fig