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Prostate cancer and melanoma experiments in mice were conducted in the BIOS+ animal facility in Bellinzona under specific pathogen-free conditions, with approval from project licences 35293, 36369 and 37480

The article says naltrexone blocks opioid receptors in the hypothalamus, removing inhibition of pro-opiomelanocortin neurons that regulate satiety, while bupropion is a norepinephrine-dopamine reuptake inhibitor that increases alertness and reduces appetite by raising dopamine and norepinephrine in the brain's appetite-control centres

Isolated RBCs are hemolyzed by addition of 1 mL of distilled H 2 O

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Most protocols recommend 30-60 minutes before intended sleep

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