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doi: 10.3389/fvets.2024.1383801 31 HeJ.ZhangP.ShenL.NiuL.TanY.ChenL.et al

The LR reaction was employed to generate the final expression construct ( pTol2-mlc2:LMNA(WT):pA/CG ), and correct colonies were selected after colony PCR and plasmid PCR

However, it did not have a significant effect on chromosomal fragility that was already present

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Mediation analyses show that early (24week) declines are only modestly weightmediated (18%31%), whereas by 72 weeks the reductions are largely weightmediated (57%87%), indicating an initial partly weightindependent signal that becomes predominantly weightlinked with sustained treatment [34]