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Immunoblotting Protein samples in 1x NuPage LDS sample buffer, 25 mM DTT (or 50 mM TCEP pH 7.0) were loaded on to SDS PAGE gels (Invitrogen, XP04205) and transferred to nitrocellulose membranes

Cadmium induces reactive oxygen species generation and lipid peroxidation in cortical neurons in culture

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In saline, APP treatment also induced significant changes in NO, total NO, and ROS contents in a treatment time-dependent manner, with their concentrations reaching 90.55 15.90 M (Fig