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These cell lines were maintained in Dulbeccos Modified Eagles Medium (DMEM) (Thermo Scientific, #12100061)low glucose for HepG2 (Thermo Scientific, #31600091), supplemented with 1 % Penicillin/Streptomycin (P/S) and 10 % Fetal bovine serum (FBS) (Natocor)

We have previously noted that the peripheral long-term subcutaneous administration of exendin-4 decreased food intake and induced weight loss in both obese and lean control Zucker rats [15]

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