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A mixture of 200 L substrate application solution, 20 L of enzyme working fluid, and 20 L of enzyme solution was incubated at 37C for 20 min, and measured using spectrophotometry at 450 nm

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They found significant reduction of SOD activities and serum levels of zinc, copper and manganese in healthy pregnant women compared to non-pregnant women with further reduction in RM patients

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E., Puri, V., Lee, K