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The retinal tissues were disrupted using a Sonic Dismembrator (FisherBrand Model 50) set at 30 in the amplitude dial for 15 s in ice-water bath (at 4 C), followed by centrifugation at 20,800 x g for 10 min at 4 C to remove particulate material 28

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The study concludes that DSIPs activating action on oxidative phosphorylation and its preventive effects under hypoxia provide a strong basis for understanding its previously reported stress-protective and antioxidant actions in vivo

Selenium and vitamin E also play supportive roles in glutathione function

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