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Ethics approval and consent to participate All animal experiments were undertaken in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals, with the approval of the Ethics Committee of the Second Affiliated Hospital, Zhejiang University School of Medicine (Permit number: 2024-260)

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All studies were RCTs and conducted in Western, Eastern, and Middle Eastern countries

doi: 10.1089/rej.2014.1624 16

Next day, invert the vial several times or gently pipette the solution up and down before use