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HEK293T cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Invitrogen, Carlsbad, CA, #12800082) with 10% Fetal Bovine Serum (FBS) (Invitrogen, Carlsbad, CA, #10099-141) supplemented with 100 units/mL penicillin and streptomycin (Invitrogen, Carlsbad, CA, #15140122)

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We are now in the early 1980srecombinant DNA technology has enabled molecular cloning of GLP-1 and GLP-2