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ROS play an important role in the regulation of many physiological processes by participating in intracellular signaling [16,20], but they can also cause serious damage to biomolecules via lipid peroxidation (autoxidation)
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The final formulation was filtered through a 0.22 m filter (FF372-10pcs, Beyotime, Shanghai, China) and adjusted to a final concentration of approximately 1 mg/ml using PBS
In the ligand-bound bovine cryo-EM structures of MRP1 (Pietz et al., 2023), important residues involved both in LTC4 and macrocyclic peptide binding were identified, including K332 and H335 (forming hydrogen bonds) and L381, F385, and F594 (forming hydrophobic contacts)
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