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The entire ileum was then cut into 1 mm 3 fragments, total fragments were transferred to centrifuge tubes and washed three times in PBS at 50 g for 3 min, followed by incubation in washing medium containing collagenase type I and hyaluronidase for 25 min at 37C

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In vivo , B7-H3.CAR-T cells encoding either CD28 or 4-1BB equally controlled tumor growth and prolonged survival in 50% of treated mice as compared to controls

Longer protocols (3-6 months) required

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