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Studies have uncovered differentially methylated profiles in paternal sperm DNA that is linked with the risk of ASD at birth [102], and the usage of a psychoactive agent, which is also associated with the risk of ASD at birth, can alter DNA methylation patterns on maternally imprinted and ASD candidate genes in the sperm [103]

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We generated the Sox9-CreER mice by integrating IRES-CreERT2-SV40pA cassettes into the 3' UTR of the endogenous mouse Sox9 gene, before the aacatggaggacgattggagaatc sequence via Cas9/RNA mediated gene targeting in zygotes

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They are supplied in one vial because they are frequently examined in combination this reflects how they are studied, and does not imply a combined effect, which remains an open research question