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Folic acid use is not advised

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B12 plays a crucial role in melanin synthesis , and its deficiency might disrupt this process, leading to hyperpigmentation

Using vented needles or introducing air displacement volume into the vial during withdrawal reduces the negative pressure that causes coring, extending both mechanical vial integrity and solution sterility

At the end of the experiment hECs were lysed in DW supplemented with Triton X-100 detergent (10 mg/ml)