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"Vitamin B12 Supplementation: Preventing Onset and Improving Prognosis of Depression"
Does the needle puncture affect storage life
Baruah, P
Due to the abundance of endogenous biotinylated proteins, especially carboxylases that use biotin as a cofactor (such as POD-2/acetyl-CoA carboxylase, PCCA-1/propionyl-CoA carboxylase, PYC-1/pyruvate carboxylase, and MCCC-1/methylcrotonyl-CoA carboxylase), which can reduce TurboID sensitivity, in 2022, the authors added a C-terminal His10 tag to these genes and subsequently removed them from the worm lysate using immobilized metal affinity chromatography
This means a single reconstituted vial can be accessed multiple times over hours or even days, reducing waste and improving operational efficiency in busy clinical environments
