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10.1042/bj3050471 33 CeliaC.CilurzoF.TrapassoE.CoscoD.FrestaM.PaolinoD

Dynamic glucose-stimulated insulin secretion in primary murine islets After isolation and overnight incubation in RPMI (10% foetal bovine serum (FBS), 1% penicillin/streptomycin), 75 islets were handpicked and placed into chambers containing 2.7 mM glucose KrebsRinger phosphate-HEPES (KRPH) buffer (140 mM NaCl, 4.7 mM KCl, 1.5 mM CaCl 2 , 1 mM NaH 2 PO4, 1 mM MgSO 4 , 2 mM NaHCO 3 , 5 mM HEPES and 0.1% FA-free BSA (pH 7.4)) with 100 l of Bio-Gel P-4 Media (Bio-Rad)

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Igarashi A, Hansen BB, Langer J, Tavella F, Collings H, Davies N, et al

Nuzum, PharmD, BCACP, CDE The chronic use of proton pump inhibitors, such as omeprazole (Prilosec), is fairly common in medical practice especially for gastroesophageal reflux disease (GERD) or in patients at increased risk of peptic ulcer disease (PUD)