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For the reporter assay, cells were transfected with a Gaussia luciferase (GLUC) plasmid containing either the human SIRT1 promoter (HRPM35661-PG02, Genecopoeia, USA) or the mouse HK2 promoter (MPRM42494-PG02, Genecopoeia) sequence, along with a Renilla luciferase plasmid

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Current NICE guidance does not recommend glutathione supplementation for non-alcoholic fatty liver disease (NAFLD) and it is not part of standard NHS treatment protocols

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