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Cells were plated into 96-well plates and routinely cultured

When disc material herniates, it triggers an inflammatory cascade involving cytokines, prostaglandins, and other chemical mediators

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Assessment of paw swelling and clinical score The CIA mice were administered 100 L of PBS, MCMs, SP@M (4 10 7 cells/mL), SP (4 10 7 cells/mL), and MTX (5 mg/kg) intravenously every three days for a total of 10 times