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Prior to dialysate analysis, a standard curve consisted of a series of known dopamine concentrations was established for every experiment

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Wakefulness time measurement To evaluate the impact of Pichia pastoris secreted recombinant peptides on insomnia, we analyzed the increase in wakefulness time among different groups of mice: Control, Model, GABA, DSIP-CBBBP, DSIP, and CBBBP

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