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The animals were allowed to acclimate to their new environment over the course of 5 days, followed by random assignment to an experimental treatment group

Because its a water-soluble vitamin, any excess that isnt used should be flushed from the body and not pose too much risk (not the case with fat-soluble vitamins like vitamin A)

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References Neelakantan H, et al

It is worth checking the volume, clarity, the 0.9% preservative concentration, integrity of the closure, batch number and storage conditions

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