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Obesity is a chronic, relapsing, and progressive disease with complex causes, including genetic, metabolic, sociocultural, behavioral, and environmental factors [1]

Real-time cell analysis for viability Real-time cell analysis (RTCA) was conducted using an Incucyte S3 live-cell analysis system (Sartorius AG, Germany)

Designed for GLP-1 medication users on Ozempic Wegovy Mounjaro or semaglutide

Additionally, the restriction of carbohydrates leads to reduced insulin levels and increased fat oxidation

Also, administering dexfenfluramine (which increases levels of extracellular serotonin) to ob/ob mice attenuates weight gain on a moderate-fat diet [49]

The oxidative stress induces an increase of membrane damage The extent of membrane damage was assessed by measuring the production of MDA, a widely recognized marker of lipoperoxidation whose production occurs in cells directly from a damage to the membrane structure and function