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In vitro functional analyses confirmed their mature hepatocyte characteristics: they showed high transcription and protein expression of ALB, A1AT, CYP3A4, and CYP2C9, comparable to primary human hepatocytes (PHs)

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For cell sorting, cell suspensions were stained with FITC-conjugated human Ep-CAM antibody and Alexa Fluor APC-conjugated human C-Kit for 30 minutes on ice

Histological examination showed reduced liver necrosis and restored tissue morphology in transplanted mice