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If you add 4ml to maintain the 2.5mg/ml concentration, you have twice the total volume to work through

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For measurements of NAD(H), 1330 mg of powdered placental tissue was weighed in a 1.5-ml microcentrifuge tube and 1,000 l of solvent A (40:40:20 acetonitrile:methanol:water with 0.1 M formic acid) was added

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Gut microbiota in systemic lupus erythematosus and correlation with diet and clinical manifestations